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| Titel |
Technical Note: Maximising accuracy and minimising cost of a potentiometrically regulated ocean acidification simulation system |
| VerfasserIn |
C. D. MacLeod, H. L. Doyle, K. I. Currie |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 12, no. 3 ; Nr. 12, no. 3 (2015-02-05), S.713-721 |
| Datensatznummer |
250117799
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| Publikation (Nr.) |
 copernicus.org/bg-12-713-2015.pdf |
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| Zusammenfassung |
| This article describes a potentiometric ocean acidification simulation
system which automatically regulates pH through the injection of 100%
CO2 gas into temperature-controlled seawater. The system is ideally
suited to long-term experimental studies of the effect of acidification on
biological processes involving small-bodied (10–20 mm) calcifying or
non-calcifying organisms. Using hobbyist-grade equipment, the system was
constructed for approximately USD 1200 per treatment unit (tank, pH
regulation apparatus, chiller, pump/filter unit). An overall tolerance of
±0.05 pHT units (SD) was achieved over 90 days in two acidified
treatments (7.60 and 7.40) at 12 °C using glass electrodes
calibrated with synthetic seawater buffers, thereby preventing liquid
junction error. The performance of the system was validated through the
independent calculation of pHT (12 °C) using dissolved
inorganic carbon and total alkalinity data taken from discrete acidified
seawater samples. The system was used to compare the shell growth of the
marine gastropod Zeacumantus subcarinatus infected with the trematode
parasite Maritrema novaezealandensis with that of
uninfected snails at pH levels of 7.4, 7.6, and 8.1. |
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