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| Titel |
Competitive interactions between methane- and ammonia-oxidizing bacteria modulate carbon and nitrogen cycling in paddy soil |
| VerfasserIn |
Y. Zheng, R. Huang, B. Z. Wang, P. L. E. Bodelier, Z. J. Jia |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 11, no. 12 ; Nr. 11, no. 12 (2014-06-24), S.3353-3368 |
| Datensatznummer |
250117479
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| Publikation (Nr.) |
 copernicus.org/bg-11-3353-2014.pdf |
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| Zusammenfassung |
| Pure culture studies have demonstrated that methanotrophs and ammonia
oxidizers can both carry out the oxidation of methane and ammonia. However,
the expected interactions resulting from these similarities are poorly
understood, especially in complex, natural environments. Using DNA-based
stable isotope probing and pyrosequencing of 16S rRNA and functional genes,
we report on biogeochemical and molecular evidence for growth stimulation of
methanotrophic communities by ammonium fertilization, and that methane
modulates nitrogen cycling by competitive inhibition of nitrifying
communities in a rice paddy soil. Pairwise comparison between microcosms
amended with CH4, CH4+Urea, and Urea indicated that urea
fertilization stimulated methane oxidation activity 6-fold during a 19-day
incubation period, while ammonia oxidation activity was significantly
suppressed in the presence of CH4. Pyrosequencing of the total 16S rRNA
genes revealed that urea amendment resulted in rapid growth of
Methylosarcina-like MOB, and nitrifying communities appeared to be
partially inhibited by methane. High-throughput sequencing of the
13C-labeled DNA further revealed that methane amendment resulted in
clear growth of Methylosarcina-related MOB while methane plus urea
led to an equal increase in Methylosarcina and
Methylobacter-related type Ia MOB, indicating the differential
growth requirements of representatives of these genera. An increase in
13C assimilation by microorganisms related to methanol oxidizers clearly
indicated carbon transfer from methane oxidation to other soil microbes,
which was enhanced by urea addition. The active growth of type Ia
methanotrops was significantly stimulated by urea amendment, and the
pronounced growth of methanol-oxidizing bacteria occurred in CH4-treated
microcosms only upon urea amendment. Methane addition partially inhibited the
growth of Nitrosospira and Nitrosomonas in urea-amended
microcosms, as well as growth of nitrite-oxidizing bacteria. These results
suggest that type I methanotrophs can outcompete type II methane oxidizers in
nitrogen-rich environments, rendering the interactions among methane and
ammonia oxidizers more complicated than previously appreciated. |
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