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| Titel |
Accelerated microbial-induced CaCO3 precipitation in a defined coculture of ureolytic and non-ureolytic bacteria |
| VerfasserIn |
D. Gat, M. Tsesarsky, D. Shamir, Z. Ronen |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 11, no. 10 ; Nr. 11, no. 10 (2014-05-16), S.2561-2569 |
| Datensatznummer |
250117409
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| Publikation (Nr.) |
 copernicus.org/bg-11-2561-2014.pdf |
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| Zusammenfassung |
| Microbial-induced CaCO3 precipitation (MICP) is an innovative technique
that harnesses bacterial activity for the modification of the physical
properties of soils. Since stimulation of MICP by urea hydrolysis in natural
soils is likely to be affected by interactions between ureolytic and
non-ureolytic bacteria, we designed an experiment to examine the
interactions between ureolytic and non-ureolytic bacteria and the effect of
these interactions on MICP. An artificial groundwater-based rich medium was
inoculated with two model species of bacteria, the ureolytic species Sporosarcina pasteurii and the
non-ureolytic species Bacillus subtilis. The control treatment was inoculated with a pure
culture of S. pasteurii. The following parameters were monitored during the course of
the experiment: optical density, pH, the evolution of ammonium,
dissolved calcium and dissolved inorganic carbon. The results showed that
dissolved calcium was precipitated as CaCO3 faster in the mixed culture
than in the control, despite less favorable chemical conditions in the mixed
culture, i.e., lower pH and lower CO32− concentration. B. subtilis exhibited
a considerably higher growth rate than S. pasteurii, resulting in higher density of
bacterial cells in the mixed culture. We suggest that the presence of the
non-ureolytic bacterial species, B. subtilis, accelerated the MICP process, via the
supply of nucleation sites in the form of non-ureolytic bacterial cells. |
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