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Titel |
Integrative analysis of Geobacter spp. and sulfate-reducing bacteria during uranium bioremediation |
VerfasserIn |
M. Barlett, K. Zhuang, R. Mahadevan, D. Lovley |
Medientyp |
Artikel
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Sprache |
Englisch
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ISSN |
1726-4170
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Digitales Dokument |
URL |
Erschienen |
In: Biogeosciences ; 9, no. 3 ; Nr. 9, no. 3 (2012-03-16), S.1033-1040 |
Datensatznummer |
250006842
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Publikation (Nr.) |
copernicus.org/bg-9-1033-2012.pdf |
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Zusammenfassung |
Enhancing microbial U(VI) reduction with the addition of organic electron
donors is a promising strategy for immobilizing uranium in contaminated
groundwaters, but has yet to be optimized because of a poor understanding of
the factors controlling the growth of various microbial communities during
bioremediation. In previous field trials in which acetate was added to the
subsurface, there were two distinct phases: an initial phase in which
acetate-oxidizing, U(VI)-reducing Geobacter predominated and U(VI) was effectively
reduced and a second phase in which acetate-oxidizing sulfate reducing
bacteria (SRB) predominated and U(VI) reduction was poor. The interaction of
Geobacter and SRB was investigated both in sediment incubations that mimicked in situ
bioremediation and with in silico metabolic modeling. In sediment incubations,
Geobacter grew quickly but then declined in numbers as the microbially reducible
Fe(III) was depleted whereas the SRB grow more slowly and reached dominance
after 30–40 days. Modeling predicted a similar outcome. Additional modeling
in which the relative initial percentages of the Geobacter and SRB were varied
indicated that there was little to no competitive interaction between
Geobacter and SRB when acetate was abundant. Further simulations suggested that the
addition of Fe(III) would revive the Geobacter, but have little to no effect on the
SRB. This result was confirmed experimentally. The results demonstrate that
it is possible to predict the impact of amendments on important components
of the subsurface microbial community during groundwater bioremediation. The
finding that Fe(III) availability, rather than competition with SRB, is the
key factor limiting the activity of Geobacter during in situ uranium bioremediation will aid
in the design of improved uranium bioremediation strategies. |
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