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Titel |
The subtle effects of sea water acidification on the amphipod Gammarus locusta |
VerfasserIn |
C. Hauton, T. Tyrrell, J. Williams |
Medientyp |
Artikel
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Sprache |
Englisch
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ISSN |
1726-4170
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Digitales Dokument |
URL |
Erschienen |
In: Biogeosciences ; 6, no. 8 ; Nr. 6, no. 8 (2009-08-07), S.1479-1489 |
Datensatznummer |
250003942
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Publikation (Nr.) |
copernicus.org/bg-6-1479-2009.pdf |
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Zusammenfassung |
We report an investigation of the effects of increases in pCO2 on the
survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which
has a cosmopolitan distribution in estuaries. Amphipods were reared from
juvenile to mature adult in laboratory microcosms at three different levels
of pH in nominal range 8.1–7.6. Growth rate was estimated from weekly
measures of body length. At sexual maturity the amphipods were sacrificed
and assayed for changes in the expression of genes coding for a heat shock
protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate
dehydrogenase (gapdh gene). The data show that the growth and survival of this
species is not significantly impacted by a decrease in sea water pH of up to
0.5 units. Quantitative real-time PCR analysis indicated that there was no
significant effect of growth in acidified sea water on the sustained
expression of the hsp70 gene. There was a consistent and significant increase in
the expression of the gapdh gene at a pH of ~7.5 which, when combined with
observations from other workers, suggests that metabolic changes may occur
in response to acidification. It is concluded that sensitive assays of
tissue physiology and molecular biology should be routinely employed in
future studies of the impacts of sea water acidification as subtle effects
on the physiology and metabolism of coastal marine species may be overlooked
in conventional gross "end-point" studies of organism growth or mortality. |
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