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| Titel |
Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences |
| VerfasserIn |
C. Pöhlker, J. A. Huffman, U. Pöschl |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1867-1381
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| Digitales Dokument |
URL |
| Erschienen |
In: Atmospheric Measurement Techniques ; 5, no. 1 ; Nr. 5, no. 1 (2012-01-09), S.37-71 |
| Datensatznummer |
250002308
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| Publikation (Nr.) |
 copernicus.org/amt-5-37-2012.pdf |
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| Zusammenfassung |
Primary biological aerosol particles (PBAP) are an important subset of air
particulate matter with a substantial contribution to the organic aerosol
fraction and potentially strong effects on public health and climate. Recent
progress has been made in PBAP quantification by utilizing real-time
bioaerosol detectors based on the principle that specific organic molecules
of biological origin such as proteins, coenzymes, cell wall compounds and
pigments exhibit intrinsic fluorescence. The properties of many fluorophores
have been well documented, but it is unclear which are most relevant for
detection of atmospheric PBAP. The present study provides a systematic
synthesis of literature data on potentially relevant biological
fluorophores. We analyze and discuss their relative importance for the
detection of fluorescent biological aerosol particles (FBAP) by online
instrumentation for atmospheric measurements such as the ultraviolet
aerodynamic particle sizer (UV-APS) or the wide issue bioaerosol sensor
(WIBS).
In addition, we provide new laboratory measurement data for selected
compounds using bench-top fluorescence spectroscopy. Relevant biological
materials were chosen for comparison with existing literature data and to
fill in gaps of understanding. The excitation-emission matrices (EEM)
exhibit pronounced peaks at excitation wavelengths of ~280 nm and
~360 nm, confirming the suitability of light sources used for online
detection of FBAP. They also show, however, that valuable information is
missed by instruments that do not record full emission spectra at multiple
wavelengths of excitation, and co-occurrence of multiple fluorophores within
a detected sample will likely confound detailed molecular analysis. Selected
non-biological materials were also analyzed to assess their possible
influence on FBAP detection and generally exhibit only low levels of
background-corrected fluorescent emission. This study strengthens the
hypothesis that ambient supermicron particle fluorescence in wavelength
ranges used for most FBAP instruments is likely to be dominated by
biological material and that such instrumentation is able to discriminate
between FBAP and non-biological material in many situations. More detailed
follow-up studies on single particle fluorescence are still required to
reduce these uncertainties further, however. |
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