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Titel |
Protein analysis in dissolved organic matter: What proteins from organic debris, soil leachate and surface water can tell us - a perspective |
VerfasserIn |
W. X. Schulze |
Medientyp |
Artikel
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Sprache |
Englisch
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ISSN |
1726-4170
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Digitales Dokument |
URL |
Erschienen |
In: Biogeosciences ; 2, no. 1 ; Nr. 2, no. 1 (2005-03-04), S.75-86 |
Datensatznummer |
250000380
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Publikation (Nr.) |
copernicus.org/bg-2-75-2005.pdf |
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Zusammenfassung |
Mass spectrometry based analysis of proteins is widely used to study
cellular processes in model organisms. However, it has not yet routinely
been applied in environmental research. Based on observations that protein
can readily be detected as a component of dissolved organic matter (DOM),
this article gives an example about the possible use of protein analysis in
ecology and environmental sciences focusing on different terrestrial
ecosystems. At this stage, there are two areas of interest: (1) the
identification of phylogenetic groups contributing to the environmental
protein pool, and (2) identification of the organismic origin of specific
enzymes that are important for ecosystem processes. In this paper, mass
spectrometric protein analysis was applied to identify proteins from
decomposing plant material and DOM of soil leachates and surface water samples
derived from different environments. It is concluded, that mass
spectrometric protein analysis is capable of distinguishing phylogenetic
origin of proteins from litter protein extracts, leachates of different soil
horizons, and from various sources of terrestrial surface water. Current
limitation is imposed by the limited knowledge of complete genomes of soil
organisms. The protein analysis allows to relate protein presence to
biogeochemical processes, and to identify the source organisms for specific
active enzymes. Further applications, such as in pollution research are
conceivable. In summary, the analysis of proteins opens a new area of
research between the fields of microbiology and biogeochemistry. |
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