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| Titel |
Annual distribution of allergenic fungal spores in atmospheric particulate matter in the Eastern Mediterranean; a comparative study between ergosterol and quantitative PCR analysis |
| VerfasserIn |
N. Lang-Yona, K. Dannemiller, N. Yamamoto, N. Burshtein, J. Peccia, O. Yarden, Y. Rudich |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1680-7316
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| Digitales Dokument |
URL |
| Erschienen |
In: Atmospheric Chemistry and Physics ; 12, no. 5 ; Nr. 12, no. 5 (2012-03-13), S.2681-2690 |
| Datensatznummer |
250010870
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| Publikation (Nr.) |
 copernicus.org/acp-12-2681-2012.pdf |
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| Zusammenfassung |
| Airborne fungal spores are an important fraction of atmospheric particulate
matter and are major causative agents of allergenic and infectious diseases.
Predicting the variability and species of allergy-causing fungal spores
requires detailed and reliable methods for identification and
quantification. There are diverse methods for their detection in the
atmosphere and in the indoor environments; yet, it is important to optimize
suitable methods for characterization of fungal spores in atmospheric
samples. In this study we sampled and characterized total and specific
airborne fungal spores from PM10 samples collected in Rehovot, Israel over
an entire year. The total fungal spore concentrations vary throughout the
year although the species variability was nearly the same. Seasonal
equivalent spore concentrations analyzed by real-time quantitative-PCR-based
methods were fall > winter > spring > summer. Reported concentrations
based on ergosterol analysis for the same samples were and fall > spring > winter > summer. Correlation between the two analytical methods was
found only for the spring season. These poor associations may be due to the
per-spore ergosterol variations that arise from both varying production
rates, as well as molecular degradation of ergosterol. While conversion of
genome copies to spore concentration is not yet straightforward, the
potential for improving this conversion and the ability of qPCR to identify
groups of fungi or specific species makes this method preferable for
environmental spore quantification. Identifying tools for establishing the
relation between the presence of species and the actual ability to induce
allergies is still needed in order to predict the effect on human health. |
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