 |
| Titel |
Experimental study of Human Adenoviruses interactions with clays |
| VerfasserIn |
Maria Bellou, Vasiliki Syngouna, Spyros Paparrodopoulos, Apostolos Vantarakis, Constantinos Chrysikopoulos |
| Konferenz |
EGU General Assembly 2014
|
| Medientyp |
Artikel
|
| Sprache |
Englisch
|
| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 16 (2014) |
| Datensatznummer |
250098011
|
| Publikation (Nr.) |
 EGU/EGU2014-13645.pdf |
|
|
|
|
|
| Zusammenfassung |
| Clays are used to establish low permeability liners in landfills, sewage lagoons, water
retention ponds, golf course ponds, and hazardous waste sites. Human adenoviruses (HAdVs)
are waterborne viruses which have been used as viral indicators of fecal pollution. The
objective of this study was to investigate the survival of HAdV in static and dynamic clay
systems. The clays used as a model were crystalline aluminosilicates: kaolinite and
bentonite. The adsorption and survival of HAdVs onto these clays were characterized
at two different controlled temperatures (4 and 25o C) under static and dynamic
batch conditions. Control tubes, in the absence of clay, were used to monitor virus
inactivation due to factors other than adsorption to clays (e.g. inactivation or sorption onto
the tubes walls). For both static and dynamic batch experiments, samples were
collected for a maximum period of seven days. This seven day time – period was
determined to be sufficient for the virus-clay systems to reach equilibrium. To infer the
presence of infectious HAdV particles, all samples were treated with Dnase and the
extraction of viral nucleid acid was performed using a commercial viral RNA kit.
All samples were analyzed by Real – Time PCR which was used to quantify viral
particles in clays. Samples were also tested for virus infectivity by A549 cell cultures.
Exposure time intervals in the range of seven days (0.50-144 hours) resulted in a load
reduction of 0.74 to 2.96 logs for kaolinite and a reduction of 0.89 to 2.92 for bentonite.
Furthermore, virus survival was higher onto bentonite than kaolinite (p |
|
|
|
|
|
|