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| Titel |
Detection and phylogenetic analysis of coastal bioaerosols using culture dependent and independent techniques |
| VerfasserIn |
R. Urbano, B. Palenik, C. J. Gaston, K. A. Prather |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 8, no. 2 ; Nr. 8, no. 2 (2011-02-10), S.301-309 |
| Datensatznummer |
250005443
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| Publikation (Nr.) |
 copernicus.org/bg-8-301-2011.pdf |
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| Zusammenfassung |
| Bioaerosols are emerging as important yet poorly understood players in
atmospheric processes. Microorganisms can impact atmospheric chemistry
through metabolic reactions and can potentially influence physical processes
by participating in ice nucleation and cloud droplet formation. Microbial
roles in atmospheric processes are thought to be species-specific and
potentially dependent on cell viability. Using a coastal pier monitoring
site as a sampling platform, culture-dependent (i.e. agar plates) and
culture-independent (i.e. DNA clone libraries from filters) approaches were
combined with 18S rRNA and 16S rRNA gene targeting to obtain insight into
the local atmospheric microbial composition. From 13 microbial isolates and
42 DNA library clones, a total of 55 sequences were obtained representing
four independent sampling events. Sequence analysis revealed that in these
coastal samples two fungal phyla, Ascomycota and Basidiomycota, predominate
among eukaryotes while Firmicutes and Proteobacteria predominate among
bacteria. Furthermore, our culture-dependent study verifies the viability of
microbes from all four phyla detected through our culture-independent study.
Contrary to our expectations and despite oceanic air mass sources, common
marine planktonic bacteria and phytoplankton were not typically found. The
abundance of terrestrial and marine sediment-associated microorganisms
suggests a potential importance for bioaerosols derived from beaches and/or
coastal erosion processes. |
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