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| Titel |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
| VerfasserIn |
A.-S. Roy, S. M. Gibbons, H. Schunck, S. Owens, J. G. Caporaso, M. Sperling, J. I. Nissimov, S. Romac, L. Bittner, M. Mühling, U. Riebesell, J. LaRoche, J. A. Gilbert |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 10, no. 1 ; Nr. 10, no. 1 (2013-01-29), S.555-566 |
| Datensatznummer |
250017489
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| Publikation (Nr.) |
 copernicus.org/bg-10-555-2013.pdf |
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| Zusammenfassung |
| The impact of ocean acidification and carbonation on microbial community
structure was assessed during a large-scale in situ costal pelagic mesocosm
study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm
experiment included ambient conditions (fjord) and nine mesocosms with
pCO2 levels ranging from ~145 to ~1420 μatm.
Samples for the present study were collected at ten time points (t–1, t1,
t5, t7, t12, t14, t18, t22, t26 to t28) in seven treatments
(ambient fjord (~145), 2 × ~185, ~270,
~685, ~820, ~1050 μatm) and were analysed for
"small" and "large" size fraction microbial community composition using
16S RNA (ribosomal ribonucleic acid) amplicon sequencing. This
high-throughput sequencing analysis produced ~20 000 000 16S rRNA V4
reads, which comprised 7000 OTUs. The main variables structuring these
communities were sample origins (fjord or mesocosms) and the community size
fraction (small or large size fraction). The community was significantly
different between the unenclosed fjord water and enclosed mesocosms (both
control and elevated CO2 treatments) after nutrients were added to the
mesocosms, suggesting that the addition of nutrients is the primary driver of
the change in mesocosm community structure. The relative importance of each
structuring variable depended greatly on the time at which the community was
sampled in relation to the phytoplankton bloom. The sampling strategy of
separating the small and large size fraction was the second most important
factor for community structure. When the small and large size fraction
bacteria were analysed separately at different time points, the only taxon
pCO2 was found to significantly affect were the Gammaproteobacteria
after nutrient addition. Finally, pCO2 treatment was found to be
significantly correlated (non-linear) with 15 rare taxa, most of which
increased in abundance with higher CO2. |
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