 |
| Titel |
The nonenzymatic template-directed ligation of oligonucleotides |
| VerfasserIn |
A. V. Lutay, E. L. Chernolovskaya, M. A. Zenkova, V. V. Vlassov |
| Medientyp |
Artikel
|
| Sprache |
Englisch
|
| ISSN |
1726-4170
|
| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 3, no. 3 ; Nr. 3, no. 3 (2006-06-20), S.243-249 |
| Datensatznummer |
250001056
|
| Publikation (Nr.) |
 copernicus.org/bg-3-243-2006.pdf |
|
|
|
|
|
| Zusammenfassung |
| The nonenzymatic template-directed ligation of oligonucleotides containing
2', 3'-cyclic phosphate was investigated in the presence of divalent cations.
Ligation of the oligonucleotides readily occurred in the presence of
Mg2+, Mn2+, Co2+, Zn2+, Pb2+. Efficacy of the metal
ion catalysts inversely correlated with pKa values of the metal-bound water
molecules. The intermolecular transesterification reaction yielded at least
95metal ion. Relatively high reaction yields (up to 15fragmentation to oligonucleotides with 2',3'-cyclic phosphates, followed by
reactions of those oligonucleotides could provide a source of new RNA
molecules under prebiotic conditions. |
| |
|
| Teil von |
|
|
|
|
|
|
|
|