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| Titel |
Insight into Emiliania huxleyi coccospheres by focused ion beam sectioning |
| VerfasserIn |
R. Hoffmann, C. Kirchlechner, G. Langer, A. S. Wochnik, E. Griesshaber, W. W. Schmahl, C. Scheu |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 12, no. 3 ; Nr. 12, no. 3 (2015-02-11), S.825-834 |
| Datensatznummer |
250117807
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| Publikation (Nr.) |
 copernicus.org/bg-12-825-2015.pdf |
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| Zusammenfassung |
| Coccospheres of a cultured Emiliania huxleyi clone were sampled in
the exponential growth phase and sectioned using a focused ion beam
microscope. An average of 69 sections and the corresponding secondary
electron micrographs per coccosphere provided detailed information on
coccosphere architecture. The coccospheres feature 2–3 layers on average and
20 coccoliths per cell, of which only 15 can be seen in conventional scanning
electron micrographs. The outer coccosphere diameter was positively
correlated with the number of coccolith layers. By contrast, the inner
coccosphere diameter (around 4.36 μm), and hence the cell diameter,
was quasi-constant. Coccoliths were not evenly distributed across the
coccosphere, resulting more often than not in one part of the coccosphere
displaying more coccolith layers than the other. The architectural data
allowed for the calculation of the PIC $/$ POC ratio, the density and the
sinking velocity of individual cells. The correlation of these parameters has
implications for the ongoing debate on the function of coccoliths. |
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