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| Titel |
Molecular biology techniques and applications for ocean sensing |
| VerfasserIn |
J. P. Zehr, I. Hewson, P. Moisander |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1812-0784
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| Digitales Dokument |
URL |
| Erschienen |
In: Ocean Science ; 5, no. 2 ; Nr. 5, no. 2 (2009-05-08), S.101-113 |
| Datensatznummer |
250002417
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| Publikation (Nr.) |
 copernicus.org/os-5-101-2009.pdf |
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| Zusammenfassung |
| The study of marine microorganisms using molecular biological techniques is
now widespread in the ocean sciences. These techniques target nucleic acids
which record the evolutionary history of microbes, and encode for processes
which are active in the ocean today. Molecular techniques can form the basis
of remote instrumentation sensing technologies for marine microbial diversity
and ecological function. Here we review some of the most commonly used
molecular biological techniques. These techniques include the polymerase chain reaction (PCR) and
reverse-transcriptase PCR, quantitative PCR, whole assemblage
"fingerprinting" approaches (based on nucleic acid sequence or length
heterogeneity), oligonucleotide microarrays, and high-throughput shotgun
sequencing of whole genomes and gene transcripts, which can be used to answer
biological, ecological, evolutionary and biogeochemical questions in the
ocean sciences. Moreover, molecular biological approaches may be deployed on
ocean sensor platforms and hold promise for tracking of organisms or
processes of interest in near-real time. |
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