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| Titel |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
| VerfasserIn |
N. Kip, C. Fritz, E. S. Langelaan, Y. Pan, L. Bodrossy, V. Pancotto, M. S. M. Jetten, A. J. P. Smolders, H. J. M. Camp |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 9, no. 1 ; Nr. 9, no. 1 (2012-01-04), S.47-55 |
| Datensatznummer |
250006650
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| Publikation (Nr.) |
 copernicus.org/bg-9-47-2012.pdf |
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| Zusammenfassung |
| Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs
living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a
methane filter and thereby reduce methane emissions. We investigated in situ methane
concentrations and the corresponding activity and diversity of methanotrophs
in different Sphagnum dominated bog microhabitats. In contrast to the Northern
Hemisphere peat ecosystems the temperate South American peat bogs are
dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent
comparison of the different bog microhabitats. Potential methane oxidizing
activity was found in all Sphagnum mosses sampled and a positive correlation was
found between activity and in situ methane concentrations. Substantial methane
oxidation activity (23 μmol CH4 gDW−1 day−1) was found in
pool mosses and could be correlated with higher in situ methane concentrations
(>35 μmol CH4 l−1 pore water). Little methanotrophic activity
(<0.5 μmol CH4 gDW−1 day−1) was observed in living
Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively
high (>4 μmol CH4 gDW−1 day−1) in Sphagnum litter at depths
around the water levels and rich in methane. The total bacterial community
was studied using 16S rRNA gene sequencing and the methanotrophic
communities were studied using a pmoA microarray and a complementary pmoA clone
library. The methanotrophic diversity was similar in the different habitats
of this study and comparable to the methanotrophic diversity found in peat
mosses from the Northern Hemisphere. The pmoA microarray data indicated that
both alpha- and gammaproteobacterial methanotrophs were present in all
Sphagnum mosses, even in those mosses with a low initial methane oxidation activity.
Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed
that the methanotrophic community present was viable and showed an increased
activity within 15 days. The high abundance of methanotrophic Methylocystis species in the
most active mosses suggests that these might be responsible for the bulk of
methane oxidation. |
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