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| Titel |
Transgenerational isotopic marking of carp Cyprinus carpio, L. using a 86Sr /84Sr double spike |
| VerfasserIn |
Andreas Zitek, Magdalena Cervicek, Johanna Irrgeher, Monika Horsky, Manfred Kletzl, Thomas Weismann, Thomas Prohaska |
| Konferenz |
EGU General Assembly 2013
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 15 (2013) |
| Datensatznummer |
250074875
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| Zusammenfassung |
| Transgenerational isotopic marking has been recognized recently as an effective tool for
mass marking and tracking of individual fish to their original source. Compared to
other conventional marking techniques, transgenerational marking offers several
advantages. Most importantly, it is possible to mark all offspring of one individual female
without the necessity of handling eggs or larval fish. Furthermore it is possible to vary
the concentrations of individual isotopes to obtain specific marks for individual
female fish. An enriched isotopic spike solution is usually applied to gravid female
spawners by injection into the body cavity for transgenerational marking. The isotope is
then incorporated into the central otolith region of the offspring which is known
to be built up by maternally derived material. Within this study transgenerational
marking of a typical cyprinid fish species, Cyprinus carpio, L., was tested using a
86Sr /84Sr double spike. Buffered solutions with different isotopic composition and
concentrations were administered to 4 female individuals by intraperitoneal injection
5 days before spawning, while one female was injected a blank solution. After
spawning, otoliths (Lapilli) from juvenile fish were sampled at the age of about 5
months at fish sizes between 3 and 4 cm and analyzed for their isotopic composition
by LA-ICPMS applying cross sectional line scans. Central otolith regions of the
progeny showed a shift in the natural isotope ratios for the administered isotopes.
Deconvolution of the blank corrected measurement data of the Sr isotopes was done to trace
back the original spike ratio. The different spike ratios could be well distinguished
reflecting the original composition of the spike solution. This study proved that it is
possible to create batch-specific unique transgenerational marks in otolith cores by
varying the concentrations of two naturally occurring Sr isotopes. This method has
high potential to reduce the marking effort for any application in aquaculture and
ecological research and management where the tracking of high numbers of offspring is
needed. |
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