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| Titel |
Testing sequential extraction methods for the analysis of multiple stable
isotope systems from a bone sample |
| VerfasserIn |
Elina Sahlstedt, Laura Arppe |
| Konferenz |
EGU General Assembly 2017
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| Medientyp |
Artikel
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| Sprache |
en
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 19 (2017) |
| Datensatznummer |
250148460
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| Publikation (Nr.) |
 EGU/EGU2017-12719.pdf |
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| Zusammenfassung |
| Stable isotope composition of bones, analysed either from the mineral phase (hydroxyapatite)
or from the organic phase (mainly collagen) carry important climatological and ecological
information and are therefore widely used in paleontological and archaeological research. For
the analysis of the stable isotope compositions, both of the phases, hydroxyapatite
and collagen, have their more or less well established separation and analytical
techniques. Recent development in IRMS and wet chemical extraction methods have
facilitated the analysis of very small bone fractions (500 μg or less starting material)
for PO43−-O isotope composition. However, the uniqueness and (pre-) historical
value of each archaeological and paleontological finding lead to preciously little
material available for stable isotope analyses, encouraging further development of
microanalytical methods for the use of stable isotope analyses. Here we present the first
results in developing extraction methods for combining collagen C- and N-isotope
analyses to PO43−-O-isotope analyses from a single bone sample fraction. We tested
sequential extraction starting with dilute acid demineralization and collection of
both collagen and PO43−-fractions, followed by further purification step by H2O2
(PO43−-fraction). First results show that bone sample separates as small as 2 mg
may be analysed for their δ15N, δ13C and δ18OPO4 values. The method may be
incorporated in detailed investigation of sequentially developing skeletal material
such as teeth, potentially allowing for the investigation of interannual variability in
climatological/environmental signals or investigation of the early life history of an individual. |
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