 |
| Titel |
Understanding the link between meteorology and speciated abundance of bioaerosols in an urban environment using colocated flow cytometry and real-time autofluorescence measurements |
| VerfasserIn |
Arnaldo Negron Marty, Natasha DeLeon-Rodriguez, Samantha Waters, Luke Ziemba, Bruce Anderson, Michael Bergin, Kostas Konstantinidis, Athanasios Nenes |
| Konferenz |
EGU General Assembly 2017
|
| Medientyp |
Artikel
|
| Sprache |
en
|
| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 19 (2017) |
| Datensatznummer |
250145598
|
| Publikation (Nr.) |
 EGU/EGU2017-9555.pdf |
|
|
|
|
|
| Zusammenfassung |
| The abundance and speciation of primary biological atmospheric particles (PBAP) has been
of great interest due to their potential impact on human health, cloud formation and
contribution to atmospheric nutrient deposition [1, 2]. During this study state-of-the-art
sampling techniques and protocols have been developed and combined with the speciation of
PBAP by flow cytometry (FCM). An effective FCM protocol has been developed to identify
and quantify speciated bioaerosols populations. In addition, a Wideband Integrated
Bioaerosol Sensor (WIBS) has been used to understand the temporal variability of the
PBAP, by measuring the autofluorescence of the atmospheric particles [3]. The
techniques developed here have been applied to understand the PBAP variability and
abundance in downtown Atlanta under different meteorological conditions. FCM results
show the presence of a low nucleic acid (LNA) and a high nucleic acid (HNA)
content subpopulation. The contribution of each subpopulation to the total biological
atmospheric particles (TBAP) varies depending on the predominant meteorological
conditions. Results suggest the HNA subpopulation, named fungal spores, dominates the
composition of the TBAP during humid and warm days after rain events. However, during
dry episodes the HNA subpopulation is diminished and the LNA subpopulation
dominates the composition of the TBAP in downtown Atlanta. WIBS size distribution
shifts between dry periods and humid and warm periods agreed well with the LNA
and HNA subpopulations behavior. Our finding suggests Atlanta average PBAP
concentration is around 1-8 x 104 part. /m3during Spring, where WIBS represents the lower
bound and FCM the upper bound of the quantification. Additional experiments
performed with different types of pollen, fungi and bacteria were used to better
understand the scattering and fluorescence properties of them under different growing
phases.
References:
[1] Morris, C. E., F. Conen, J. Alex Huffman, V. Phillips, U. Poschl and D. C. Sands
(2014). Bioprecipitation: a feedback cycle linking earth history, ecosystem dynamics and land
use through biological ice nucleators in the atmosphere. Glob Chang Biol 20(2):
341-351.
[2] Després, V. R., J. Alex Huffman, S. M. Burrows, C. Hoose, A. S. Safatov, G.
Buryak, J., Fröhlich-Nowoisky, W. Elbert, M. O. Andreae, U. Pöschl and R. Jaenicke
(2012). Primary biological aerosol particles in the atmosphere: a review. Tellus B
64(0).
[3] Gabey, A. M., M. W. Gallagher, J. Whitehead, J. R. Dorsey, P. H. Kaye and W. R.
Stanley (2010). Measurements and comparison of primary biological aerosol above and
below a tropical forest canopy using a dual channel fluorescence spectrometer. Atmospheric
Chemistry and Physics 10(10): 4453-4466. |
|
|
|
|
|
|