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Titel Microbes Characteristics in Groundwater Flow System in Mountainous Area
VerfasserIn Chisato Yamamoto, Maki Tsujimura, Kenji Kato, Koichi Sakakibara, Mahiro Ogawa, Ayumi Sugiyama, Kazuyo Nagaosa
Konferenz EGU General Assembly 2017
Medientyp Artikel
Sprache en
Digitales Dokument PDF
Erschienen In: GRA - Volume 19 (2017)
Datensatznummer 250140443
Publikation (Nr.) Volltext-Dokument vorhandenEGU/EGU2017-3838.pdf
 
Zusammenfassung
We focus on a possibility of microbes as a tracer for groundwater flow investigation. Some previous papers showed that the total number of prokaryotes in groundwater has correlation with depth and geology (Parkes et al., 1994; Griebler et al., 2009; Kato et al., 2012). However, there are few studies investigating both microbe characteristics and groundwater flow system. Therefore, we investigated a relationship between the total number of prokaryotes and age of spring water and groundwater. Intensive field survey was conducted at four mountainous areas, namely Mt. Fuji (volcano), a headwater at Mt. Setohachi, a headwater at River Oi and a headwater at River Nagano underlain by volcanic lava at Mt. Fuji, granite at Mt. Setohachi and sedimentary rock at River Oi and River Nagano. We collected totally 40 spring water/ groundwater samples in these mountainous areas in October 2015, August, October and November 2016 and analyzed concentration of inorganic ions, the stable isotopes of oxygen – 18, deuterium, CFCs and SF6. Also, we counted prokaryotic cells under the epifluorescence microscopy after fixation and filteration. The total number of prokaryotes in the spring water/ groundwater ranged from 1.0×102 to 7.0×103cells mL−1 at the Mt. Fuji, 1.3×104 to 2.7×105cells mL−1 at Mt. Setohachi, 3.1×104cells mL−1 at River Oi and 1.8×105 to 3.2×106cells mL−1 at River Nagano. The SF6 age of the spring water/ groundwater ranged from 8 to 64 years at Mt. Fuji, 2 to 32.5 years at Mt. Setohachi, 2.5 years at River Oi and 15 to 16 years at River Nagano. The total number of prokaryotes showed a clear negative correlation with residence time of spring water/ groundwater in all regions. Especially the prokaryotes number increased in the order of 102 cells mL−1 with decreasing of residence time in approximately 10 years in the groundwater and spring water with the age less than 15 years.