 |
| Titel |
Microbial Abundances in Salt Marsh Soils: A Molecular Approach for Small Spatial Scales |
| VerfasserIn |
Dirk Granse, Peter Mueller, Magdalena Weingartner, Stefan Hoth, Kai Jensen |
| Konferenz |
EGU General Assembly 2016
|
| Medientyp |
Artikel
|
| Sprache |
en
|
| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 18 (2016) |
| Datensatznummer |
250127281
|
| Publikation (Nr.) |
 EGU/EGU2016-7138.pdf |
|
|
|
|
|
| Zusammenfassung |
| %=================================================================
%=================================================================
% macros:
%
\newcommand{\Fungi}{\mbox{\textit{Fungi}}}
\newcommand{\Bacteria}{\mbox{\textit{Bacteria}}}
\newcommand{\GenecopiesPerDryWeightSoil}{$\cdot$10$^7$~gene-copies~g~(dry~wt)~of~soil$^{-1}$}
\newcommand{\ZBL}[0]{\mbox{e.g.,}}
\newcommand{\QPCR}[0]{\mbox{qPCR}}
\newcommand{\WE} [2]{\mbox{#1}~{#2}}
\newcommand{\RSquared}{\textit{r}$^2$}
\newcommand{\FullTitle}{ Microbial Abundances in Salt Marsh Soils: A Molecular Approach for Small Spatial Scales }
%=================================================================
%=================================================================
\title{\FullTitle}
\author{Dirk Granse}
%=========================================================================================
%=========================================================================================
%=========================================================================================
%=========================================================================================
%=========================================================================================
%=========================================================================================
\begin{document}
%=========================================================================================
%=========================================================================================
%=========================================================================================
The rate of biological decomposition greatly determines the carbon sequestration capacity of salt marshes. Microorganisms are involved in the decomposition of biomass and the rate of decomposition is supposed to be related to microbial abundance. Recent studies quantified microbial abundance by means of quantitative polymerase chain reaction (\QPCR), a method that also allows determining the microbial community structure by applying specific primers. The main microbial community structure can be determined by using primers specific for 16S rRNA (\Bacteria) and 18S rRNA (\Fungi) of the microbial DNA. However, the investigation of microbial abundance pattern at small spatial scales, such as locally varying abiotic conditions within a salt-marsh system, requires high accuracy in DNA extraction and \QPCR~methods. Furthermore, there is evidence that a single extraction may not be sufficient to reliably quantify rRNA gene copies. The aim of this study was to establish a suitable DNA extraction method and stable \QPCR~conditions for the measurement of microbial abundances in semi-terrestrial environments.
DNA was extracted from two soil samples (top \WE{5}{cm}) by using the PowerSoil DNA Extraction Kit (Mo Bio Laboratories, Inc., Carlsbad, CA) and applying a modified extraction protocol. The DNA extraction was conducted in four consecutive DNA extraction loops from three biological replicates per soil sample by reusing the PowerSoil bead tube. The number of \Fungi~and \Bacteria~rRNA gene copies of each DNA extraction loop and a pooled DNA solution (extraction loop 1 - 4) was measured by using the \QPCR~method with taxa specific primer pairs (\Bacteria: B341F, B805R; \Fungi: FR1, FF390).
The DNA yield of the replicates varied at DNA extraction loop 1 between \WE{25 and 85}{ng |
|
|
|
|
|
|