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| Titel |
Evaluation of germination, growth and ecophysiological response of Cistus monspeliensis L. in different contaminated and uncontaminated soils of the Iberian Pyrite Belt |
| VerfasserIn |
Daniel Arenas Lago, Erika S. Santos, Luisa C. Carvalho, Maria Manuela Abreu |
| Konferenz |
EGU General Assembly 2016
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| Medientyp |
Artikel
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| Sprache |
en
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 18 (2016) |
| Datensatznummer |
250127128
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| Publikation (Nr.) |
 EGU/EGU2016-6964.pdf |
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| Zusammenfassung |
| Iberian Pyrite Belt (IPB) is one of the most important volcanogenic massive sulfide ore
deposits in the world. As a result of the mine activities, many areas from the IPB
have become extreme environments with high concentrations of a wide variety
of potentially hazardous elements (PHEs) and low pH in their soils, which cause
severe contamination problems and inhibit or reduce the plant colonization and their
growth. Nevertheless, Cistus monspeliensis L. grows spontaneously in mine areas
from the IPB under these extreme conditions, which suggests that this species must
have mechanisms to adapt and defend itself against oxidative stress caused by the
high levels of PHEs. The main objectives of this study are to evaluate germination,
growth, development and ecophysiological behaviour of C. monspeliensis in different
contaminated and uncontaminated soils. For this purpose, two different assays were
conducted in potted plants in a greenhouse with C. monspeliensis seeds collected
in the São Domingos mine area (SE Portugal, IPB). In the first assay, twenty C.
monspeliensis seeds were sowed to evaluate the germination in pots (n=4) with five
different contaminated and uncontaminated soils - Uncontaminated soils: a sandy
soil (A) and a soil from Caldeirão (C) (S of Portugal), Contaminated soils: two
gossans from São Domingos mine (SD and G) and a gossan amended with an organic
corrective (GC). After one month, germination rate was evaluated. Total and available
multielemental concentrations were determined in the soils. In the second assay,
C. monspeliensis seedlings were planted in the contaminated soil GC and in the
uncontaminated soil C. After three months of growth, plants were harvested and
shoots were separated from roots. Plant height, fresh biomass and multielemental
concentration in shoots were quantified. Pigments (chlorophylls, anthocyanins and
carotenoids), glutathione, ascorbate, H2O2 and the activities of several key antioxidative
enzymes were also quantified in shoots. In the first assay there were no significant
differences in the results obtained between germination rates of C. monspeliensis in
contaminated and uncontaminated soils (%): 46.3 ± 8.5 (A), 46.3 ± 8.5 (C), 47.5
± 24.7 (SD), 41.3 ± 10.3 (G) and 36.3 ± 18.4 (GC). Available concentrations
(mg/kg) of PHEs in the soils (second assay) were significantly higher in GC (Zn: 3-5;
As: 2-3; Pb: 2-3) than in C (Zn, As, Pb: < 1). Significant differences were also
found in the growth and development of C. monspeliensis plants. Plants height,
leaf size and plant dry biomass were higher in individuals from C soil than from
GC soil, although toxicity symptoms were not observed in any of them. Cistus
monspeliensis growing in GC soil showed higher H2O2 contents and lower levels of
pigments than plants growing in C soil. Furthermore, plants growing in GC soil
triggered defence mechanisms against oxidative stress, in the form of increased
antioxidative enzyme activity. As a general conclusion, these results reveal that C.
monspeliensis is adapted to unfavourable environments with high concentrations of
PHEs, adjusting its tolerance mechanisms at the metabolic and physiological levels. |
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