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| Titel |
Metaproteogenomics reveals the soil microbial communities active in nutrient
cycling processes under different tree species |
| VerfasserIn |
Katharina Maria Keiblinger, Jacynthe Masse, Daniela Zühlke, Katharina Riedel, Sophie Zechmeister-Boltenstern, Cindy E. Prescott, Sue Grayston |
| Konferenz |
EGU General Assembly 2016
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| Medientyp |
Artikel
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| Sprache |
en
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 18 (2016) |
| Datensatznummer |
250125254
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| Publikation (Nr.) |
 EGU/EGU2016-4813.pdf |
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| Zusammenfassung |
| Tree species exert strong effects on microbial communities in litter and soil and may alter
rates of soil processes fundamental to nutrient cycling and carbon fluxes (Prescott and
Grayston 2013). However, the influence of tree species on decomposition processes are still
contradictory and poorly understood. An understanding of the mechanisms underlying plant
influences on soil processes is important for our ability to predict ecosystem response to
altered global/environmental conditions.
In order to link microbial community structure and function to forest-floor nutrient
cycling processes, we sampled forest floors under western redcedar (Thuja plicata),
Douglas-fir (Pseudotsuga menziesii) and Sitka spruce (Picea sitchensis) grown in
nutrient-poor sites in common garden experiments on Vancouver island (Canada). We
measured forest-floor total N, total C, initial NH4+ and NO3− concentrations, DOC, Cmic
and Nmic. Gross rates of ammonification and NH4+ consumption were measured using the
15N pool-dilution method. Organic carbon quality was assessed through FTIR analyses.
Microbial community structure was analysed by a metaproteogenomic approach using 16S
and ITS amplification and sequencing with MiSeq platform. Proteins were extracted and
peptides characterized via LC-MS/MS on a Velos Orbitrap to assess the active microbial
community.
Different microbial communities were active under the three tree species and variation in
process rates were observed and will be discussed. This research provides new insights on
microbial processes during organic matter decomposition. The metaproteogenomic approach
enables us to investigate these changes with respect to possible effects on soil C-storage at
even finer taxonomic resolution. |
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