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| Titel |
Metaproteome of the viral concentrates from the deep chlorophyll maximum of the South China Sea |
| VerfasserIn |
Zhang-Xian Xie, Feng Chen, Shu-Feng Zhang, Ming-Hua Wang, Hao Zhang, Ling-Fen Kong, Min-Han Dai, Hua-Sheng Hong, Lin Lin, Da-Zhi Wang |
| Konferenz |
EGU General Assembly 2016
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| Medientyp |
Artikel
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| Sprache |
en
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 18 (2016) |
| Datensatznummer |
250122285
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| Publikation (Nr.) |
 EGU/EGU2016-1282.pdf |
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| Zusammenfassung |
| Viral concentrates (VCs) have been commonly used for studying viral diversity, viral
metagenomics and virus-host interactions in the natural ecosystem. However, the protein
characteristics of VCs have not been explored. Here, we applied shotgun proteomics to
characterize the proteins of VCs collected from the oligotrophic deep chlorophyll maximum
of the South China Sea. We found that 34% of the identified proteins were assigned
to the viruses, mainly being those of SAR11 related bacteria, cyanobacteria and
picophytoeukaryotes. The remaining 66% were non-viral proteins mostly originating
from diverse bacteria, such as SAR324, SAR11 and the Alteromonadales, and were
functionally dominated by transport, translation, sulfur metabolism and one-carbon
metabolism. Among the non-viral proteins, 28% were extracellular proteins and 10%
were identified exclusively in the VCs, suggesting that non-viral entities might
exist in the VCs. This study demonstrated that metaproteomics provides a valuable
avenue to explore not only the diversity and structure of a viral community but also
the novel ecological functions affiliated with microbes in the natural environment. |
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