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| Titel |
Methodological interference of biochar in the determination of extracellular enzyme activities in composting samples |
| VerfasserIn |
K. Jindo, K. Matsumoto, C. García Izquierdo, T. Sonoki, M. A. Sanchez-Monedero |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1869-9510
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| Digitales Dokument |
URL |
| Erschienen |
In: Solid Earth ; 5, no. 2 ; Nr. 5, no. 2 (2014-07-29), S.713-719 |
| Datensatznummer |
250115321
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| Publikation (Nr.) |
 copernicus.org/se-5-713-2014.pdf |
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| Zusammenfassung |
| Biochar application has received increasing attention as a means to trap
recalcitrant carbon and enhance soil fertility. Hydrolytic enzymatic assays,
such as β-glucosidase and phosphatase activities, are used for the
assessment of soil quality and composting process, which are based on use of
p-nitrophenol (PNP) derivatives as substrate. However, sorption capacity of
biochar can interfere with colorimetric determination of the hydrolysed PNP,
either by the sorption of the substrate or the reaction product of
hydrolysis into biochar surface. The aim of the present work is to study the
biochar sorption capacity for PNP in biochar-blended composting mixtures in
order to assess its impact on the estimation of the colorimetric-based
enzymatic assays. A retention test was conducted by adding a solution of
known amounts of PNP in universal buffer solution (pH = 5, 6.5 and 11,
corresponding to the β-glucosidase, acid and alkaline phosphatase
activity assays, respectively), in samples taken at the initial stage and
after maturation stage from four different composting piles (two manure
composting piles; PM: poultry manure, CM: cow manure and two other similar
piles containing 10% of additional biochar (PM + B, CM + B)). The results
show that biochar-blended composts (PM + B, CM + B) generally exhibited low
enzymatic activities, compared to manure compost without biochar (PM, CM).
In terms of the difference between the initial and maturation stage of
composting process, the PNP retention in biochar was shown higher at
maturation stage, caused most probably by an enlarged proportion of biochar
inside compost mixture after the selective degradation of easily
decomposable organic matter. TThe retention of PNP on biochar was influenced by pH
dependency of sorption capacity of biochar and/or PNP solubility, since PNP was
more efficiently retained by biochar at low pH values (5 and 6.5) than at high pH values (11). |
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