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| Titel |
Identification of Ina proteins from Fusarium acuminatum |
| VerfasserIn |
Jan Frederik Scheel, Anna Theresa Kunert, Ulrich Pöschl, Janine Fröhlich-Nowoisky |
| Konferenz |
EGU General Assembly 2015
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 17 (2015) |
| Datensatznummer |
250110436
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| Publikation (Nr.) |
 EGU/EGU2015-10431.pdf |
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| Zusammenfassung |
| Freezing of water above -36Ë C is based on ice nucleation activity (INA) mediated by ice
nucleators (IN) which can be of various origins. Beside mineral IN, biological particles are a
potentially important source of atmospheric IN. The best-known biological IN are common
plant-associated bacteria. The IN activity of these bacteria is induced by a surface protein on
the outer cell membrane, which is fully characterized. In contrast, much less is known about
the nature of fungal IN.
The fungal genus Fusarium is widely spread throughout the earth. It belongs to the
Ascomycota and is one of the most severe fungal pathogens. It can affect a variety of
organisms from plants to animals including humans. INA of Fusarium was already
described about 30 years ago and INA of Fusarium as well as other fungal genera is
assumed to be mediated by proteins or at least to contain a proteinaceous compound.
Although many efforts were made the precise INA machinery of Fusarium and
other fungal species including the proteins and their corresponding genes remain
unidentified.
In this study preparations from living fungal samples of F. acuminatum were fractionated
by liquid chromatography and IN active fractions were identified by freezing assays.
SDS-page and de novo sequencing by mass spectrometry were used to identify the primary
structure of the protein.
Preliminary results show that the INA protein of F. acuminatum is contained in the early
size exclusion chromatography fractions indicating a high molecular size. Moreover we could
identify a single protein band from IN active fractions at 130-145 kDa corresponding to sizes
of IN proteins from bacterial species. To our knowledge this is for the first time an isolation
of a single protein from in vivo samples, which can be assigned as IN active from Fusarium. |
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