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| Titel |
Quantification of 16S gene and its relation with the CO2 emission and soil properties in areas under management of sugarcane (Saccharum spp.) |
| VerfasserIn |
Mara Regina Moitinho, Elton da Silva Bicalho, Daniel De Bortoli Teixeira, Newton Jr. La Scala |
| Konferenz |
EGU General Assembly 2015
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 17 (2015) |
| Datensatznummer |
250108272
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| Publikation (Nr.) |
 EGU/EGU2015-8019.pdf |
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| Zusammenfassung |
| A diversity of microorganisms has an essential role in the recycling of soil chemical
elements, controlling, for example, the dynamics of carbon decomposition and stabilization,
and consequently the patterns of soil CO2 emission. In this sense, the objectives of this study
were: (i) to estimate and compare the genetic diversity of microorganisms in soils under
different sugarcane (Saccharum spp.) managements using molecular techniques based on
metagenomic studies, and (ii) investigate the relationship of soil CO2 emission (FCO2) with
microbiological results and soil chemical and physical properties in the evaluated
managements. This study was conducted in agricultural areas located in southern Brazil, in
which the following sugarcane managements were used: green and burned residues
management, a sugarcane area under reform, and a native forest (used as a reference of the
original soil condition). FCO2, soil temperature, and soil moisture were measured
over 10 days, and at the end of the measurements soil samples were taken in order
to determine the physical and chemical soil properties. The determination of the
diversity of soil microorganisms was carried out by means of molecular techniques
based on 16S rRNA gene sequencing. The highest mean value for FCO2 (3.25 μmol
m-2s-1) was observed in the sugarcane area under reform, and the lowest values
(1.85 and 1.27 μmol m-2s-1) were observed respectively in the green residue
management and native forest areas. This same pattern was also observed when the
16S gene was quantified. In this case, the largest number of copies of this gene
was found in the sugarcane area under reform (4.3x1010 copies of 16S rRNA gene
per gram of dry soil), and its smallest number of copies was found in the green
residues management area (1.7x1010 copies of 16S rRNA gene per gram of dry soil).
The largest number of copies of the 16S gene associated to the highest values of
FCO2, both observed in the sugarcane area under reform, could be related to the
biomass and microbial activity increasing, which are directly related to a good soil
fertility conditions and agricultural practices (liming and harrowing), such as the
area under reform evaluated in this study. This fact was confirmed by the positive
correlation (r = 0.98; p < 0.0001) observed between FCO2 and the number of copies
of the 16S gene. FCO2 was also related to the soil moisture (r = -0.22; p < 0.05)
in the area of green residue management, and to the soil temperature (r = 0.28; p |
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