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| Titel |
Spatial distribution of enzyme activities along the root and in the rhizosphere of different plants |
| VerfasserIn |
Bahar S. Razavi, Mohsen Zarebanadkouki, Evgenia Blagodatskaya, Yakov Kuzyakov |
| Konferenz |
EGU General Assembly 2015
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 17 (2015) |
| Datensatznummer |
250102120
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| Publikation (Nr.) |
 EGU/EGU2015-1400.pdf |
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| Zusammenfassung |
| Extracellular enzymes are important for decomposition of many biological macromolecules
abundant in soil such as cellulose, hemicelluloses and proteins. Activities of enzymes
produced by both plant roots and microbes are the primary biological drivers of organic
matter decomposition and nutrient cycling. So far acquisition of in situ data about local
activity of different enzymes in soil has been challenged. That is why there is an urgent
needÂin spatially explicit methods such as 2-D zymography to determine the variation of
enzymes along the roots in different plants. Here, we developed further the zymography
technique in order to quantitatively visualize the enzyme activities (Spohn and Kuzyakov,
2013), with a better spatial resolution
We grew Maize (Zea mays L.) and Lentil (Lens culinaris) in rhizoboxes under optimum
conditions for 21 days to study spatial distribution of enzyme activity in soil and along roots.
We visualized the 2D distribution of the activity of three enzymes:β-glucosidase, leucine
amino peptidase and phosphatase, using fluorogenically labelled substrates. Spatial resolution
of fluorescent images was improved by direct application of a substrate saturated membrane
to the soil-root system.
The newly-developed direct zymography shows different pattern of spatial distribution of
enzyme activity along roots and soil of different plants. We observed a uniform
distribution of enzyme activities along the root system of Lentil. However, root system
of Maize demonstrated inhomogeneity of enzyme activities. The apical part of
an individual root (root tip) in maize showed the highest activity. The activity of
all enzymes was the highest at vicinity of the roots and it decreased towards the
bulk soil. Spatial patterns of enzyme activities as a function of distance from the
root surface were enzyme specific, with highest extension for phosphatase. We
conclude that improved zymography is promising in situ technique to analyze,
visualize and quantify spatial distribution of enzyme activities in the rhizosphere
hotspots.Â
References
Spohn, M., Kuzyakov, Y., 2013. Phosphorus mineralization can be driven by microbial
need for carbon. Soil Biology & Biochemistry 61: 69-75 |
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