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| Titel |
Understanding aquatic microbial processes using EEM's and in-situ fluorescence sensors |
| VerfasserIn |
Bethany Fox, John Attridge, Cathy Rushworth, Tim Cox, Alexandre Anesio, Darren Reynolds |
| Konferenz |
EGU General Assembly 2015
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 17 (2015) |
| Datensatznummer |
250101669
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| Publikation (Nr.) |
 EGU/EGU2015-857.pdf |
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| Zusammenfassung |
| The diverse origin of dissolved organic matter (DOM) in aquatic systems is well documented
within the literature. Previous literature indicates that coloured dissolved organic matter
(CDOM) is, in part, transformed by aquatic microbial processes, and that dissolved organic
material derived from a microbial origin exhibits tryptophan-like fluorescence. However, this
phenomenon is not fully understood and very little data is available within the current
literature. The overall aim of our work is to reveal the microbial-CDOM interactions that give
rise to the observed tryptophan-like fluorescence. The work reported here investigates the
microbial processes that occur within freshwater aquatic samples, as defined by the
biochemical oxygen demand (BOD) test, as a function of the T1 peak (λex/em
280/330-370 nm). A series of standard water samples were prepared using glucose,
glutamic acid, BOD dilution water and a bacterial seed (Cole-Parmer BOD microbe
capsules). Samples were spiked with CDOM (derived from an environmental water
body) and subjected to time resolved BOD analysis and as excitation-emission
fluorescence spectroscopy. All EEM spectral data was interrogated using parallel factor
analysis (PARAFAC) in an attempt to determine the presence and dominance (relative
intensities) of the CDOM-related and T1-related fluorophores within the samples. In-situ
fluorescence sensors (Chelsea Technologies Group Ltd.) were also used to monitor the T1
fluorescence peak (UviLux Tryptophan) and the CDOM fluorescence peak (UviLux
CDOM) during experiments. Tryptophan-like fluorescence was observed (albeit
transient) in both spiked and un-spiked standard water samples. By furthering our
understanding of aquatic organic matter fluorescence, its origin, transformation, fate and
interaction with aquatic microbiological processes, we aim to inform the design of a new
generation in-situ fluorescence sensor for the monitoring of aquatic ecosystem health. |
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