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Titel |
Geomicrobial characterization of a 60 m long permafrost core from Svalbard |
VerfasserIn |
Siren Fromreide, Pål Tore Mørkved, Graham Lewis Gilbert, Hanne H. Christiansen, Laila Reigstad |
Konferenz |
EGU General Assembly 2014
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Medientyp |
Artikel
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Sprache |
Englisch
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Digitales Dokument |
PDF |
Erschienen |
In: GRA - Volume 16 (2014) |
Datensatznummer |
250096768
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Publikation (Nr.) |
EGU/EGU2014-12286.pdf |
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Zusammenfassung |
In connection with a planned CO2 storage pilot project in the Arctic, a 60 m long permafrost
core was drilled in Adventdalen, Svalbard. The on-shore drilling was performed through
mainly marine and deltafront sediments, ending at the bedrock. The core has undergone
detailed analyses of sedimentary stratigraphy, age, as well as the permafrost ice and carbon
content at The University Centre in Svalbard (UNIS), and at the Center for Permafrost
(CENPERM), Copenhagen.
The main aim of the study presented here is to link the geochemical properties of the
permafrost to the microbial community structure and its potential functions. As little is
known about microbial life in permafrost at such depths this study will contribute to the
understanding of these inaccessible ecosystems.
A baseline geomicrobial description of 7 different depths in the 3 – 60 m interval of the
permafrost core was done by culture independent methods such as 16S rRNA amplicon 454
pyrosequencing and functional and ribosomal gene quantifications. Additionally,
geochemical analyses of the extracted pore water have been performed, as well as
measurements of carbon content and major elements.
The enumeration of the total prokaryotic community indicated similar numbers of
bacteria and archaea down to approximately 50 m depth, while below this depth there
was a dominance of archaeal cells. The bacterial 16S rRNA copy numbers ranged
between 108 copies per gram sediment at 3 m depth to 104 copies per gram at the
bedrock. Concerning the archaeal cells, the 16S rRNA copy numbers per gram
sediment were in the range of 107 at the top of the core, ending at 105in the top of the
bedrock.
Detection and quantification of selected functional marker genes indicated high numbers
of sulphate reducing bacteria at certain sediment depths, and a significant potential for
microbial methanogenic activity throughout the core. Correlations studies between
geochemical data and microbial community composition are currently ongoing. |
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