 |
| Titel |
Long-term fertilization of organic manure led to the succession of Bacillus community in an alluvial-aquic soil |
| VerfasserIn |
Ruirui Chen, Xiangui Lin, Youzhi Feng, Junli Hu, Ruirui Wang |
| Konferenz |
EGU General Assembly 2014
|
| Medientyp |
Artikel
|
| Sprache |
Englisch
|
| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 16 (2014) |
| Datensatznummer |
250090985
|
| Publikation (Nr.) |
 EGU/EGU2014-5248.pdf |
|
|
|
|
|
| Zusammenfassung |
| Long-term fertilization inevitably influences soil physic-chemical and biological properties.
Our previous studies with a long-term fertilization experiment on an alluvial-aquic have
revealed that specific Bacillus spp. was observed in organic manure-fertilized soils. The
current study investigated the effects of long-term fertilization on the succession of Bacillus
community in soils and their functions. The experiment included three fertilizer treatments:
organic manure (OM), mineral fertilizers (NPK) and the control (without fertilizers). The
results showed that long-term application of chemical fertilizers didn’t increase the quantity
of soil microbial population as much as organic fertilizers did, but it played an important role
in maintaining the diversity and community structure of indigenous Bacilli. Correspondingly,
long-term application of organic manure significantly increased the quantity while
significantly decreased the diversity of Bacilli community. The ratio of Bacilli/bacteria was
more constant in OM treatment than NPK indicating the stability of the response to
long-term organic fertilizers. PCR-DGGE and clone library revealed the succession of
Bacillus community after long-term application of organic manure and the dominant
Bacillus spp occurred in the treatmen OM was Bacillus asahii. Our results also proved
that Bacillus asahii was not derived from exogenous organic manure, but one of
indigenous bacteria in the soil. Bacillus asahii was induced by the substrate after
the application of organic manure, and gradually evolved into dominant Bacillus
after 4 to 5 years. With an enzyme assay test of pure species and a soil incubation
experiment, we came to a preliminary judgment, that the dominant Bacillus asahii
didn’t significantly influence the decomposition rate of cellulose and protein in
the soil, but it promoted the decomposition of lipids, and could also improve the
transformation process from fresh organic matter to humus. Applied organic manure led to
the succession of soil microbial community, as a response, the changed microbial
community and their activities influenced the turnover of exogenous and native soil
organic matter, as well as the residuals of decomposition and microbial metabolisms. |
|
|
|
|
|
|