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| Titel |
Vertical dynamics of the aquifer microbial community associated with groundwater chemistry in the artificial recharge site in Korea |
| VerfasserIn |
Hee Sun Moon, Sung Pil Hyun, Boa Kim, Doyun Shin, Kyoochul Ha |
| Konferenz |
EGU General Assembly 2014
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 16 (2014) |
| Datensatznummer |
250089165
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| Publikation (Nr.) |
 EGU/EGU2014-3358.pdf |
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| Zusammenfassung |
| Artificial groundwater recharge offers an opportunity to better manage groundwater
resources by storing water in aquifers and increasing the amount of groundwater
available for abstraction during high demand periods. It is important to understand
the linkage of microbial ecology to groundwater chemistry to assess changes in
groundwater quality caused by artificial groundwater recharge. In this study, we
investigated how the structure and diversity of this subsurface microbial community
correlates with and impacts upon groundwater chemistry. Groundwater samples at
two different depths (10 and 33 m) were collected from three monitoring wells
(MLW 1, MLW 2 and MLW 3) in the artificial groundwater recharge demonstration
site in Changwon, Korea. The groundwater samples were filtered with 0.45 um
membrane filters and then used for the anion and cation analysis. A 4L of each
groundwater sample was immediately filtered with 0.2 um membrane filters and the filters
were used for DNA extraction using Fast DNA Spin Kit for soil (MP Bio, USA).
Further molecular work processes including pyrosequencing were carried out at
Chunlab, Inc. (Seoul, Korea). Pyrosequencing results showed all major phyla were
OD 1, OD3, and OD 11 in shallow groundwater samples while Proteobacteria
(β-proteobacteria and δ-proteobacteria) and Bacterioidetes were dominant phyla in deep
groundwater. The Shannon diversity index indicated that the microbial community
was much more diverse in shallow groundwater than in deep groundwater. Heat
map and hierarchical cluster analysis based on the relative abundance of OTUs at
genus level showed a clear distinction between shallow and deep groundwater.
Differences in the vertical community structure were driven by the major species such
as Sufuicurvum sp., Pseudomonas sp., Acidiferrobacter sp., Gallionella sp., and
Ferribacterium sp. The results show that several distinct factors such as iron and
sulfate concentration control the vertical composition of microbial communities
in this aquifer. In conclusion, iron and sulfur chemistry combined with microbial
community structure is useful in predicting groundwater ecology and groundwater quality
changes caused by the surface water injection in the artificial recharge of aquifers. |
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