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| Titel |
Effect of soil structure on the growth of bacteria in soil quantified using CARD-FISH |
| VerfasserIn |
Archana Juyal, Thilo Eickhorst, Ruth Falconer, Wilfred Otten |
| Konferenz |
EGU General Assembly 2014
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 16 (2014) |
| Datensatznummer |
250086495
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| Publikation (Nr.) |
 EGU/EGU2014-375.pdf |
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| Zusammenfassung |
| It has been reported that compaction of soil due to use of heavy machinery has
resulted in the reduction of crop yield. Compaction affects the physical properties of
soil such as bulk density, soil strength and porosity. This causes an alteration in
the soil structure which limits the mobility of nutrients, water and air infiltration
and root penetration in soil. Several studies have been conducted to explore the
effect of soil compaction on plant growth and development. However, there is scant
information on the effect of soil compaction on the microbial community and its activities
in soil. Understanding the effect of soil compaction on microbial community is
essential as microbial activities are very sensitive to abrupt environmental changes in
soil.
Therefore, the aim of this work was to investigate the effect of soil structure on growth of
bacteria in soil. The bulk density of soil was used as a soil physical parameter to quantify the
effect of soil compaction. To detect and quantify bacteria in soil the method of catalyzed
reporter deposition-fluorescence in situ hybridization (CARD-FISH) was used. This
technique results in high intensity fluorescent signals which make it easy to quantify
bacteria against high levels of autofluorescence emitted by soil particles and organic
matter.
In this study, bacterial strains Pseudomonas fluorescens SBW25 and Bacillus subtilis
DSM10 were used. Soils of aggregate size 2-1mm were packed at five different bulk densities
in polyethylene rings (4.25 cm3).The soil rings were sampled at four different days.
Results showed that the total number of bacteria counts was reduced significantly
(P |
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