 |
| Titel |
Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel Bathymodiolus azoricus |
| VerfasserIn |
E. Martins, A. Queiroz, R. Serrão Santos, R. Bettencourt |
| Medientyp |
Artikel
|
| Sprache |
Englisch
|
| ISSN |
1726-4170
|
| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 10, no. 11 ; Nr. 10, no. 11 (2013-11-14), S.7279-7291 |
| Datensatznummer |
250085414
|
| Publikation (Nr.) |
 copernicus.org/bg-10-7279-2013.pdf |
|
|
|
|
|
| Zusammenfassung |
The deep-sea hydrothermal vent mussel Bathymodiolus azoricus lives in a natural environment
characterised by extreme conditions of hydrostatic pressure, temperature,
pH, high concentrations of heavy metals, methane and hydrogen sulphide. The
deep-sea vent biological systems represent thus the opportunity to study and
provide new insights into the basic physiological principles that govern the
defense mechanisms in vent animals and to understand how they cope with
microbial infections. Hence, the importance of understanding this animal's
innate defense mechanisms, by examining its differential immune gene
expressions toward different pathogenic agents. In the present study, B. azoricus
mussels were infected with single suspensions of marine bacterial pathogens,
consisting of Vibrio splendidus, Vibrio alginolyticus, or Vibrio anguillarum, and a pool of these Vibrio bacteria. Flavobacterium suspensions were also used
as a non-pathogenic bacterium. Gene expression analyses were carried out using gill
samples from infected animals by means of quantitative-Polymerase Chain
Reaction aimed at targeting several immune genes. We also performed SDS-PAGE
protein analyses from the same gill tissues.
We concluded that there are different levels of immune gene expression
between the 12 h to 24 h exposure times to various bacterial suspensions.
Our results from qPCR demonstrated a general pattern of gene expression,
decreasing from 12 h over 24 h post-infection. Among the bacteria tested,
Flavobacterium is the bacterium inducing the highest gene expression level in 12 h
post-infections animals. The 24 h infected animals revealed, however,
greater gene expression levels, using V. splendidus as the infectious agent. The SDS-PAGE
analysis also pointed at protein profile differences between 12 h and 24 h,
particularly evident for proteins of 18–20 KDa molecular mass, where most
dissimilarity was found. Multivariate analyses demonstrated that immune
genes, as well as experimental infections, clustered in discrete groups in
accordance with the gene expression patterns induced by bacterial pathogens. |
| |
|
| Teil von |
|
|
|
|
|
|
|
|