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| Titel |
Technical Note: Controlled experimental aquarium system for multi-stressor investigation of carbonate chemistry, oxygen saturation, and temperature |
| VerfasserIn |
E. E. Bockmon, C. A. Frieder, M. O. Navarro, L. A. White-Kershek, A. G. Dickson |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 10, no. 9 ; Nr. 10, no. 9 (2013-09-11), S.5967-5975 |
| Datensatznummer |
250085329
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| Publikation (Nr.) |
 copernicus.org/bg-10-5967-2013.pdf |
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| Zusammenfassung |
| As the field of ocean acidification has grown, researchers have increasingly
turned to laboratory experiments to understand the impacts of increased
CO2 on marine organisms. However, other changes such as ocean warming
and deoxygenation are occurring concurrently with the increasing CO2
concentrations, complicating the understanding of the impacts of
anthropogenic changes on organisms. This experimental aquarium design allows
for independent regulation of CO2 concentration, O2 levels, and
temperature in a controlled environment to study the impacts of multiple
stressors. The system has the flexibility for a wide range of treatment
chemistry, seawater volumes, and study organisms. Control of the seawater
chemistry is achieved by equilibration of a chosen gas mixture with seawater
using a Liqui-Cel® membrane contactor.
Included as examples, two experiments performed using the system have shown
control of CO2 at values between approximately 500 and 1400 μatm
and O2 at values from 80 to 240 μmol kg−1. Temperature has
been maintained to 0.5 °C or better in the range of
10–17 °C. On a weeklong timescale, the system has achieved
variability in pH of less than 0.007 pH units and in oxygen concentration of
less than 3.5 μmol kg−1. Longer experiments, over a month in
duration, have been completed with control to better than 0.08 pH units and
13 μmol kg−1 O2. The ability to study the impacts of
multiple stressors in the laboratory simultaneously, as well as
independently, will be an important part of understanding the response of
marine organisms to a high-CO2 world. |
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