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| Titel |
Comparison of the elemental composition of different hard parts (otoliths, scales, fin rays, vertebrae and eye lenses) of freshwater fish using ICPMS |
| VerfasserIn |
Leo Kirchmaier, Johanna Irrgeher, Thomas Prohaska, Andreas Zitek |
| Konferenz |
EGU General Assembly 2013
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 15 (2013) |
| Datensatznummer |
250076038
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| Zusammenfassung |
| Elemental and isotopic fingerprints of different hard parts of fish are a powerful tool to gain
life history information of individual fish. Most of these structures, like otoliths, scales, fin
rays and vertebrae show incremental growth, allowing for a time resolved analysis of this
information using e.g. laser ablation-inductively coupled plasma mass spectrometry
(LA-ICPMS). Scales and fin-rays serve as important non-lethal alternatives to otoliths.
Additional structures without incremental growth such as eye lenses might contain
complementary information. However, there is a lack of solid matrix-matched reference
materials that are needed to quantify elemental concentrations using LA-ICPMS. In this study
we determined the elemental composition (“elemental fingerprint”) of otoliths, scales,
fin rays, vertebrae and eye lenses from freshwater fish of the Danube catchment
using microwave-assisted digestion and solution based inductively coupled plasma
mass spectrometry in order to gain information on the natural concentration ranges
of various elements of interest in such samples. The investigation has focused on
macro (Ca, Mg and P), minor and trace elements (Ba, Cu, Mn, Pb, Sr and Zn).
Method validation was performed using the following certified reference materials
(CRMs): riverine water (NRC SLRS-5), bone ash (NIST SRM 1400), bone meal (NIST
SRM 1486) and fish otolith (NRC FEBS-1). Significant differences in elemental
concentrations among different hard parts analyzed were found suggesting that
different structures might be suited for a particular research purpose e.g. tracing
environmental pollution. Based on this work, the preparation of in-house certified
matrix-matched reference materials (co-precipitated hydroxyapatite calibration standards
pressed to pellets) is established allowing for the quantification of the elemental
concentration on a time resolved level in different hard parts by direct solid sampling via
laser. |
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