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| Titel |
Influence of Bentonite Particles on the Transport and Deposition Behavior of Bacteria in Porous Media |
| VerfasserIn |
Haiyan Yang, Meiping Tong, Hyunjung Kim |
| Konferenz |
EGU General Assembly 2013
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 15 (2013) |
| Datensatznummer |
250073434
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| Zusammenfassung |
| The influence of clay particles on the transport and deposition behavior of bacteria in
irregular quartz sand was examined by direct comparison of both breakthrough curves and
retained profiles with clay particles in bacteria suspension versus those without clay particles.
Two representative cell types, Gram-negative strain E.coli DH5α and Gram-positive strain
Bacillus subtilis were utilized to systematically determine the influence of clay particles
(bentonite) on cell transport behavior. Packed column experiments for both cell types
were conducted in both NaCl (5 and 25 mM ionic strengths) and CaCl2 (5 mM
ionic strength) solutions at pH 6.0. The breakthrough plateaus with bentonite in
solutions (30 mg L-1 and 50 mg L-1) were lower than those without bentonite for
both cell types under all examined conditions, indicating that bentonite in solutions
decreased cell transport in porous media regardless of cell types (Gram-negative or
Gram-positive) and solution chemistry (ionic strength and ion valence). The enhanced
cell deposition with bentonite particles was mainly observed at segments near to
column inlet, retained profiles for both cell types with bentonite particles therefore
were steeper relative to those without bentonite. The increased cell deposition with
bentonite observed in NaCl solutions was mainly attributed to the co-deposition of
bacteria with bentonite particles whereas, in addition to co-deposition of bacteria
with bentonite, the bacteria-bentonite-bacteria cluster formed in suspensions also
contributed to the increased deposition of bacteria with bentonite in CaCl2 solution. |
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