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| Titel |
UV-laser microdissection system - A novel approach for the preparation of high-resolution stable isotope records (δ¹³C/δ¹⁸O) from tree rings |
| VerfasserIn |
Karina Schollaen, Gerhard Helle |
| Konferenz |
EGU General Assembly 2013
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 15 (2013) |
| Datensatznummer |
250072532
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| Zusammenfassung |
| Intra-annual stable isotope (δ13C and δ18O) studies of tree rings at various incremental
resolutions have been attempting to extract valuable seasonal climatic and environmental
information or assessing plant ecophysiological processes. For preparing high-resolution
isotope samples normally wood segments or cores are mechanically divided in radial
direction or cut in tangential direction. After mechanical dissection, wood samples are ground
to a fine powder and either cellulose is extracted or bulk wood samples are analyzed. Here,
we present a novel approach for the preparation of high-resolution stable isotope records
from tree rings using an UV-laser microdissection system. Firstly, tree-ring cellulose is
directly extracted from wholewood cross-sections largely leaving the wood anatomical
structure intact and saving time as compared to the classical procedure. Secondly,
micro-samples from cellulose cross-sections are dissected with an UV-Laser dissection
microscope. Tissues of interest from cellulose cross-sections are identified and
marked precisely with a screen-pen and dissected via an UV-laser beam. Dissected
cellulose segments were automatically collected in capsules and are prepared for
stable isotope (δ13C and δ18O) analysis. The new techniques facilitate inter- and
intra-annual isotope analysis on tree-ring and open various possibilities for comparisons
with wood anatomy in plant eco-physiological studies. We describe the design and
the handling of this novel methodology and discuss advantages and constraints
given by the example of intra-annual oxygen isotope analysis on tropical trees. |
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