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| Titel |
Detection and Identification of Free-living Amoeba from Environmental Water in Taiwan by PCR Method |
| VerfasserIn |
H. F. Tsai, B. M. Hsu, K. H. Huang, C. Y. She, P. M. Kao, S. M. Shen, S. F. Tseng, J. S. Chen |
| Konferenz |
EGU General Assembly 2012
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 14 (2012) |
| Datensatznummer |
250065361
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| Zusammenfassung |
| Acanthamoeba, Naegleria, Balamuthia and Hartmannella all belong to free-living amoebae
that are present ubiquitously in the environment including water, soil, and air. Free-living
amoebae are parasites which can infect humans and can lead to serious illness and even death.
The aim of this study is to investigate the presence of free-living amoebae in aquatic
environment in Taiwan, and to compare the differences between Acanthamoeba and
Naegleria in diverse cultivation methods and conditions. In this study, we used molecular
method by PCR amplification with specific primers to analyze the occurrence of free-living
amoebae. We collected 176 samples from environmental water including drinking water
treatment plants, stream water, and hot spring recreational areas in Taiwan. Based on the
results of PCR, 43 water samples (24.4%) were detected positive for free-living amoebae.
The most common Acanthamoeba genotype isolated from samples including T2,
T4, T5, T12, and T15. N. australiensis and N. lovaniensis were also identified by
molecular biology techniques. Furthermore, we found that both Acanthamoeba
and Naegleria can be cultured by PYG in 30Ë C, but not all free-living amoebae
can be isolated and enriched by using storage-cultivation method. Because of the
widespread presence of Acanthamoeba and Naegleria in aquatic environments, the water
quality and safety of aquatic environments should be more conscious in Taiwan and
worldwide.
Keywords: free-living amoebae; Acanthamoeba; Naegleria; Balamuthia; Hartmannella;
PCR |
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