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| Titel |
Transgenerational marking of a freshwater fish species, Salmo trutta f.f. L., using an enriched 84Sr spike solution |
| VerfasserIn |
Andreas Zitek, Johanna Irrgeher, Albert Jagsch, Manfred Kletzl, Thomas Weismann, Thomas Prohaska |
| Konferenz |
EGU General Assembly 2011
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 13 (2011) |
| Datensatznummer |
250046500
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| Zusammenfassung |
| Transgenerational marking with enriched isotopes has been proven to be a suitable method
for marking otoliths of fish larvae via the transmission of the maternal isotope signal to study
dispersal and migration in aquatic ecosystems. However, so far the method has been mainly
applied to marine fish species by using barium isotopes only. Herein, we present for the first
time the results of maternal transmission of an artificially introduced strontium isotope signal
(84Sr) on the example of a typical European freshwater fish species, the brown trout, Salmo
trutta f.f. L..
Brown trout mother fish were intraperitoneally injected with 1, 2.5 and 5 ml of 84Sr
solutions resulting in doses of 15.4, 38.6 and 77.1 μg 84Sr kg-1 fish, respectively, with
strontium bearing the advantage of being non-toxic. Fish were then stripped, and the resulting
progeny reared in a hatchery for about a year before sampling.
Strontium ratios were measured over the otoliths’ cross sections of both the mother fish
and the offspring by doing line scans using laser ablation - multiple collector - inductively
coupled plasma - mass spectrometry (LA-MC-ICP-MS).
The analysis of the maternal otoliths showed a clear increase of the 84Sr/86Sr
ratio at the edge of the otolith independent of the amount of 84Sr solution injected.
Otolith cores of the juveniles showed a significant elevation of 84Sr/86Sr whereas
control fish showed stable 84Sr/86Sr ratios along the entire cross section of the
otoliths.
The results proved that transgenerational marking with enriched strontium isotopes
represents a new and effective method for mass-marking of freshwater fish larvae in the field
without the necessity of handling the eggs, allowing for studying natural dispersal of fish in
river systems.
Future experiments comprise spiking experiments using other enriched strontium isotopes
(e.g. 86Sr) and other fish species such as the carp (Cyprinus carpio L.), representing cyprinid
species with very small egg sizes. |
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