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Titel |
Transport processes and mutual interactions of three bacterial strains in saturated porous media |
VerfasserIn |
Christine Stumpp, John R. Lawrence, M. Jim Hendry, Pitor Maloszewski |
Konferenz |
EGU General Assembly 2010
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Medientyp |
Artikel
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Sprache |
Englisch
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Digitales Dokument |
PDF |
Erschienen |
In: GRA - Volume 12 (2010) |
Datensatznummer |
250042701
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Zusammenfassung |
Transport processes of the bacterial strains Klebsiella oxytoca, Burkholderia cepacia G4PR-1
and Pseudomonas sp #5 were investigated in saturated column experiments to study the
differences in transport characteristics and the mutual interactions of these strains during
transport. Soil column experiments (114 mm long x 33 mm in diameter) were conducted with
constant water velocities (3.9-5.7 cm/h) through a medium to coarse grained silica sand. All
experiments were performed in freshly packed columns in quadruplicate. Chloride was used
as tracer to determine the mean transit time, dispersivity and flow rate. It was injected as a
pulse into the columns together with the bacterial strains suspended in artificial
groundwater medium. In the first setup, each strain was investigated alone. In the second
setup, transport processes were performed injecting two strains simultaneously.
Finally, the transport characteristics were studied in successive experiments when one
bacterium was resident on the sand grains prior to the introduction of the second
strain.
In all experiments the peak C/Co bacterial concentrations were attenuated with respect to
the conservative tracer chloride and a well defined tailing was observed. A one dimensional
mathematical model for advective-dispersive transport that accounts for irreversible and
reversible sorption was used to analyze the bacterial breakthrough curves and tailing patterns.
It was shown that the sorption parameters were different for the three strains that can be
explained by the properties of the bacteria. For the species Klebsiella oxytoca and
Burkholderia cepacia G4PR-the transport parameters were mostly in the same range
independent of the experimental setup. However, Pseudomonas sp #5, which is a motile
bacterium, showed differences in the breakthrough curves and sorption parameters during the
experiments. The simultaneous and successive experiments indicated an influence on the
reversible sorption processes when another strain was present during the transport processes. |
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