 |
| Titel |
Deep microbial life in the Altmark natural gas reservoir: baseline characterization prior CO2 injection |
| VerfasserIn |
Daria Morozova, Mina Shaheed, Andrea Vieth, Martin Krüger, Dagmar Kock, Hilke Würdemann |
| Konferenz |
EGU General Assembly 2010
|
| Medientyp |
Artikel
|
| Sprache |
Englisch
|
| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 12 (2010) |
| Datensatznummer |
250039925
|
|
|
|
|
|
| Zusammenfassung |
| Within the framework of the CLEAN project (CO2 Largescale Enhanced gas recovery in the
Altmark Natural gas field) technical basics with special emphasis on process monitoring are
explored by injecting CO2 into a gas reservoir. Our study focuses on the investigation
of the in-situ microbial community of the Rotliegend natural gas reservoir in the
Altmark, located south of the city Salzwedel, Germany. In order to characterize
the microbial life in the extreme habitat we aim to localize and identify microbes
including their metabolism influencing the creation and dissolution of minerals.
The ability of microorganisms to speed up dissolution and formation of minerals
might result in changes of the local permeability and the long-term safety of CO2
storage. However, geology, structure and chemistry of the reservoir rock and the cap
rock as well as interaction with saline formation water and natural gases and the
injected CO2 affect the microbial community composition and activity. The reservoir
located at the depth of about 3500m, is characterised by high salinity fluid and
temperatures up to 127- C. It represents an extreme environment for microbial
life and therefore the main focus is on hyperthermophilic, halophilic anaerobic
microorganisms. In consequence of the injection of large amounts of CO2 in the course
of a commercial EGR (Enhanced Gas Recovery) the environmental conditions
(e.g. pH, temperature, pressure and solubility of minerals) for the autochthonous
microorganisms will change. Genetic profiling of amplified 16S rRNA genes are
applied for detecting structural changes in the community by using PCR- SSCP
(PCR–Single-Strand-Conformation Polymorphism) and DGGE (Denaturing Gradient
Gel Electrophoresis). First results of the baseline survey indicate the presence of
microorganisms similar to representatives from other saline, hot, anoxic, deep environments.
However, due to the hypersaline and hyperthermophilic reservoir conditions, cell
numbers are low, so that the quantification of those microorganisms as well as the
determination of microbial activity was not yet possible. Microbial monitoring
methods have to be further developed to study microbial activities under these extreme
conditions to access their influence on the EGR technique and on enhancing the
long term safety of the process by fixation of carbon dioxide by precipitation of
carbonates.
We would like to thank GDF SUEZ for providing the data for the Rotliegend reservoir,
sample material and enabling sampling campaigns. The CLEAN project is funded by the
German Federal Ministry of Education and Research (BMBF) in the frame of the
Geotechnologien Program. |
|
|
|
|
|
|