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| Titel |
Monitoring the alkane monooxygenase gene alkB in different soil interfaces during plant litter degradation of C3 and C4 plants |
| VerfasserIn |
S. Schulz, J. C. Munch, M. Schloter |
| Konferenz |
EGU General Assembly 2009
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| Medientyp |
Artikel
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| Sprache |
Englisch
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| Digitales Dokument |
PDF |
| Erschienen |
In: GRA - Volume 11 (2009) |
| Datensatznummer |
250019450
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| Zusammenfassung |
| Hydrocarbons like n-alkanes are ubiquitous in the environment as a result of anthropogenic
contamination (e.g. oil spills) as well as a part of an ecosystem’s biomass. For example
n-alkanes become released during plant litter degradation; consequently they become a high
abundant carbon source for microorganism. One possibility for the prokaryotic hydrocarbon
metabolisation is an aerobic degradation pathway where the initial step is catalysed by the
membrane bound alkane monooxygenase alkB.
We analysed the influence of alkanes on the abundance of the alkB gene in different
interfaces of the litter-soil system during the degradation of maize and pea litter. Therefore
soil samples of a sandy and a loamy soil have been incubated with straw of maize
and pea plants up to 30 weeks with constant soil moisture and temperature. Using
quantitative real-time PCR we were able to monitor the changes of the abundance and the
expression rates of alkB. In our experiments we focused on the straw layer, the
litter/soil interface and the soil 1 cm below this interface (bulk soil). Our results
clearly demonstrate time and space dependent abundance patterns of alkB genes and
transcripts in the different layers studied, which are additionally shaped by the soil type
used. |
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