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| Titel |
Diversity and abundance of n-alkane-degrading bacteria in the near-surface soils of a Chinese onshore oil and gas field |
| VerfasserIn |
K. Xu, Y. Tang, C. Ren, K. Zhao, Y. Sun |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 10, no. 3 ; Nr. 10, no. 3 (2013-03-25), S.2041-2048 |
| Datensatznummer |
250018170
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| Publikation (Nr.) |
 copernicus.org/bg-10-2041-2013.pdf |
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| Zusammenfassung |
| Alkane-degrading bacteria have long been used as an important biological
indicator for oil and gas prospecting, but their ecological characteristics
in hydrocarbon microseep habitats are still poorly understood. In this study,
the diversity and abundance of n-alkane-degrading bacterial community in
the near-surface soils of a Chinese onshore oil and gas field were
investigated using molecular techniques. Terminal restriction fragment length
polymorphism (T-RFLP) analyses in combination with cloning and sequencing of
alkB genes revealed that Gram-negative genotypes
(Alcanivorax and Acinetobacter) dominated
n-alkane-degrading bacterial communities in the near-surface soils of oil
and gas reservoirs, while the dominant microbial communities were
Gram-positive bacteria (Mycobacterium and Rhodococcus) in
background soil. Real-time quantitative polymerase chain reaction (PCR)
results furthermore showed that the abundance of alkB genes
increased substantially in the surface soils above oil and gas reservoirs
even though only low or undetectable concentrations of hydrocarbons were
measured in these soils. The results of this study implicate that trace
amounts of volatile hydrocarbons migrate from oil and gas reservoirs, and
likely result in the changes of microbial communities in the near-surface
soil. |
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