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| Titel |
Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay |
| VerfasserIn |
L. E. King, R. J. Weber |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1867-1381
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| Digitales Dokument |
URL |
| Erschienen |
In: Atmospheric Measurement Techniques ; 6, no. 7 ; Nr. 6, no. 7 (2013-07-11), S.1647-1658 |
| Datensatznummer |
250017918
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| Publikation (Nr.) |
 copernicus.org/amt-6-1647-2013.pdf |
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| Zusammenfassung |
| An online, semi-continuous instrument to measure fine particle (PM2.5)
reactive oxygen species (ROS) was developed based on the fluorescent probe
2'7'-dichlorofluorescin (DCFH). Parameters that influence probe response
were first characterized to develop an optimal method for use in a field
instrument. The online method used a mist chamber scrubber to collect total
(gas plus particle) ROS components (ROSt) alternating with gas
phase ROS (ROSg) by means of an inline filter. Particle phase ROS
(ROSp) was determined by the difference between ROSt
and ROSg. The instrument was deployed in urban Atlanta, Georgia,
USA, and at a rural site during various seasons. Concentrations from the
online instrument generally agreed well with those from an intensive filter
measurement of ROSp. Concentrations of the ROSp
measurements made with this instrument were lower than reported in other
studies, often below the instrument's average limit of detection
(0.15 nmol H2O2 equivalents m−3). Mean ROSp
concentrations were 0.26 nmol H2O2 equivalents m−3 at the
Atlanta urban sites compared to 0.14 nmol H2O2
equivalents m−3 at the rural site. |
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