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| Titel |
Distribution of typical denitrifying functional genes and diversity of the nirS-encoding bacterial community related to environmental characteristics of river sediments |
| VerfasserIn |
S. Huang, C. Chen, X. Yang, Q. Wu, R. Zhang |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 8, no. 10 ; Nr. 8, no. 10 (2011-10-31), S.3041-3051 |
| Datensatznummer |
250006172
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| Publikation (Nr.) |
 copernicus.org/bg-8-3041-2011.pdf |
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| Zusammenfassung |
| Denitrification in river sediments leads to nitrate removal from the aquatic
system; therefore, it is necessary to understand functional diversity of
denitrifier communities in the system. Sediment samples (0–25 cm depth) were
collected from three typical locations along the Pearl River. The real-time
PCR approach was used to measure the abundance of nitrate (narG), nitrite
(nirS, nirK and nrfA), and nitrous oxide (nosZ) reductase genes from the sediment samples.
Assemblages of nirS, nirK and nosZ indicated that complete denitrification occurred in
sediment cores, with the greatest number of gene copies from 5–15 cm depth.
Dissimilatory nitrate reduction appeared to be important below 15 cm depth,
based on increasing gene copies of narG and nrfA with sediment depth. There was a
close match (78–94 %) between the nirS sequences recovered from the Pearl
River sediment and those detected in estuarine and marine sediments as well
as active sludge, suggesting that the nitrogen source in the Pearl River
sediment was affected by domestic sewage inputs and irregular tides.
Canonical correspondence analysis indicated that the spatial distribution of
denitrifying bacteria was highly correlated with dissolved inorganic
nitrogen (including NH4+, NO2− and NO3−)
concentrations in sediment. It was concluded that the difference in
dissolved inorganic nitrogen concentrations along the sediment profile
influenced the distribution of denitrifying genes and the nirS-encoding
denitrifier community in the river sediment. In addition, a variety of novel
denitrifying bacteria were revealed in the river sediment. |
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