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| Titel |
Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by 13C-fingerprinting |
| VerfasserIn |
V. Riou, S. Bouillon, R. Serrão Santos, F. Dehairs, A. Colaço |
| Medientyp |
Artikel
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| Sprache |
Englisch
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| ISSN |
1726-4170
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| Digitales Dokument |
URL |
| Erschienen |
In: Biogeosciences ; 7, no. 9 ; Nr. 7, no. 9 (2010-09-02), S.2591-2600 |
| Datensatznummer |
250004959
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| Publikation (Nr.) |
 copernicus.org/bg-7-2591-2010.pdf |
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| Zusammenfassung |
| Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of
their energy requirements are met via an endosymbiotic association with
chemolithotrophic and methanotrophic bacteria. In an effort to describe
phenotypic characteristics of the two bacterial endosymbionts and to assess
their ability to assimilate CO2, CH4 and multi-carbon compounds,
we performed experiments in aquaria using 13C-labeled NaHCO3 (in
the presence of H2S), CH4 or amino-acids and traced the
incorporation of 13C into total and phospholipid fatty acids (tFA and
PLFA, respectively). 14:0; 15:0; 16:0; 16:1(n − 7)c+t; 18:1(n − 13)c+t and (n − 7)c+t;
20:1(n − 7); 20:2(n − 9,15); 18:3(n − 7) and (n − 5,10,13) PLFA were labeled in the
presence of H13CO3− (+H2S) and 13CH4, while
the 12:0 compound became labeled only in the presence of
H13CO3− (+H2S). In contrast, the 17:0; 18:0; 16:1(n − 9);
16:1(n − 8) and (n − 6); 18:1(n − 8); and 18:2(n − 7) PLFA were only labeled in the
presence of 13CH4. Some of these symbiont-specific fatty acids
also appeared to be labeled in mussel gill tFA when incubated with
13C-enriched amino acids, and so were mussel-specific fatty acids such
as 22:2(n − 7,15). Our results provide experimental evidence for the potential
of specific fatty acid markers to distinguish between the two endosymbiotic
bacteria, shedding new light on C1 and multi-carbon compound metabolic
pathways in B. azoricus and its symbionts. |
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